Figure 1. Generation of mice carrying the CX₃CR1^CreER allele.

(A) Schematic of targeting strategy used for knock-in of CreER-IRES-YFP at the CX₃CR1 locus. (B) Southern blot analysis of AflII digested genomic DNA from untargeted (WT), CX₃CR1^CreER targeted, or CX₃CR1^CreER mice after deletion of the neomycin resistance sequence. (C) Coronal sections of motor cortex from P45 CX₃CR1^CreER mice stained for EYFP, Iba1, and NeuN. (D) Coronal sections of motor cortex from mice of the indicated genotypes and treatments (scale bar = 100 μm).