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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Environ Toxicol. 2013 Nov 26;30(4):490–499. doi: 10.1002/tox.21926

Fig. 2 (A–E). Dose- and time- response increase in Gadd45α expression in PW cells exposed to Nano-Co.

Fig. 2 (A–E)

PW cells were treated with 0, 1.25, 2.5, 5, and 10 μg/ml of Nano-Co or Nano-TiO2 for 6 h (A–C), or with 5 μg/ml of Nano-Co for 3, 6, and 12 h (D–E). Cells without treatment were used as the control. Gadd45α mRNA level was determined by real-time PCR (A) while protein level was determined by Western blot (B–E). (A) shows the real-time PCR results as mean ± SD of three experiments with triplicate in each experiment. (B and D) show the results of a single Western blot experiment. (C and E) represent normalized band densitometry readings averaged from three independent experiments ± SD of Western results. * Significant difference as compared with the control, p<0.05; # Significant difference as compared with the same dose of Nano-TiO2-treated group, p<0.05.