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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Environ Toxicol. 2013 Nov 26;30(4):490–499. doi: 10.1002/tox.21926

Fig. 3 (A–D). Dose- and time- response increase in HIF-1α expression in PW cells exposed to Nano-Co.

Fig. 3 (A–D)

PW cells were treated with 0, 1.25, 2.5, 5, and 10 μg/ml of Nano-Co for 6 h (A and B) or treated with 5 μg/ml of Nano-Co for 3, 6, and 12 h (C and D). Cells without particle treatment were used as the control. (A and C) show the results of a single Western blot experiment. (B and D) represent normalized band densitometry readings averaged from three independent experiments ± SD of Western results. * Significant difference as compared with the control, p<0.05.