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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Environ Toxicol. 2013 Nov 26;30(4):490–499. doi: 10.1002/tox.21926

Fig. 5 (A–D). Dose-response induction of Gadd45α protein expression in HIF-1α (+/+) cells (A and B) exposed to Nano-Co, but not in HIF-1α (−/−) cells (C and D).

Fig. 5 (A–D)

Cells were treated with 1.25, 2.5, 5, and 10 μg/ml of Nano-Co for 6 h. Cells without particle treatment were used as the control. Gadd45α protein level was measured by Western blot. (A and C) show the results of a single Western blot experiment. (B and D) represent normalized band densitometry readings averaged from three independent experiments ± SD of Western results. * Significant difference as compared with the control, p<0.05.