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. 2014 May 1;3:221. doi: 10.1186/2193-1801-3-221

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© Hara et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Immunohistochemistry of R ⁵⁸ LAP-DP in fibrotic liver tissues in CCl ₄ -treated mice using R58 antibody. Liver tissues from CCl₄-treated and control (olive oil administrated) mice were stained by HE (a, d)vand Sirius Red ( b, e), and then immunostained with R58 antibody (c, f). Scale bar = 50 μm. The percentage of fibrotic regions and R58 positive areas were calculated from three fields each from five sections (5 mice) (total 15 fields) and described as average ± SE under the corresponding panels. A total of 16 mice were evaluated and representative results are shown.