Table 1.
Concentrations of the compounds present in the essential oil of Rosmarinus officinalis L. obtained by different methods
| No | Compounds | RT 1a (min) | RT 2b (min) | Concentration (mg mL−1) | |||
|---|---|---|---|---|---|---|---|
| 45 min | 210 min | 10 min | 75 min | ||||
| Hydrodistillation | MAHD | ||||||
| 1 | Tricyclene | 7.910 | 13.401 | 0.903 | 0.837 | 1.177 | 1.523 |
| 2 | α-thujene | 8.095 | 13.549 | 0.841 | 0.718 | 0.991 | 0.868 |
| 3 | α -pinene | 8.350 | 13.889 | 79.625 | 85.710 | 120.695 | 84.323 |
| 4 | Camphene | 8.874 | 14.534 | 20.404 | 25.464 | 30.442 | 24.211 |
| 5 | Verbenenec | 9.101 | 14.810 | – | 0.281 | – | – |
| 6 | Sabinenec | 9.869 | 15.629 | – | – | 0.248 | 0.224 |
| 7 | β-pinene | 9.970 | 15.760 | 27.696 | 32.847 | 41.549 | 33.161 |
| 8 | 1-octen-3-ol | 10.176 | 16.096 | 2.118 | 1.423 | 1.787 | 1.332 |
| 9 | β -myrcene | 10.605 | 16.291 | 9.108 | 11.579 | 14.622 | 11.234 |
| 10 | α -phellandrene | 11.119 | 16.949 | 1.300 | 1.799 | 1.817 | 1.643 |
| 11 | 3-carene | 11.378 | 17.213 | 0.000 | 0.742 | 0.661 | 0.000 |
| 12 | α -terpinene | 11.648 | 17.502 | 3.059 | 4.846 | 4.324 | 4.279 |
| 13 | P-cymene | 12.014 | 17.875 | 7.556 | 11.010 | 9.536 | 10.046 |
| 14 | Limonene | – | 18.080 | 4.138 | 15.151 | 16.454 | 14.826 |
| 15 | 1,8-cineole | 12.363 | 18.234 | 538.797 | 436.875 | 409.754 | 450.188 |
| 16 | Cis-Ocimene | 12.644 | 18.909 | – | – | – | – |
| 17 | γ-terpinene | 13.502 | 19.407 | 3.521 | 6.219 | 4.764 | 5.582 |
| 18 | Terpinolene | 14.826 | 20.771 | 1.763 | 5.332 | 2.447 | 3.081 |
| 19 | Linalool | 15.413 | 21.214 | 13.520 | 14.352 | 12.248 | 13.583 |
| 20 | Fenchol | 15.975 | 21.955 | 1.532 | 2.342 | 1.049 | 1.509 |
| 21 | Campholaldehydec | 16.589 | 22.528 | 0.591 | 0.696 | 0.297 | 0.586 |
| 22 | Camphor | 17.399 | 23.420 | 139.592 | 153.297 | 149.583 | 167.174 |
| 23 | Isopulegol | 17.939 | 23.903 | 1.074 | 0.887 | 1.059 | 0.843 |
| 24 | Pinocamphone/isopinocamphone | 18.146 | 24.140 | 1.030 | 0.727 | 0.843 | 0.764 |
| 25 | Pinocarvone/trans-pinocarvone | 18.236 | 24.252 | 0.299 | – | 0.344 | – |
| 26 | Borneol | 18.352 | 24.330 | 21.488 | 35.582 | 28.329 | 39.672 |
| 27 | 4-terpineol | 18.892 | 24.837 | 7.568 | 10.497 | 8.153 | 11.294 |
| 28 | p-cymene-8-olc | 19.295 | 25.143 | 0.223 | 0.893 | 0.857 | 1.145 |
| 29 | α-terpineol | 19.539 | 25.401 | 18.104 | 36.869 | 26.356 | 41.003 |
| 30 | Myrtenolc | 19.814 | 25.937 | – | 0.401 | 0.199 | 0.886 |
| 31 | Verbenone | 20.418 | 26.315 | 1.389 | 2.939 | 2.133 | 3.273 |
| 32 | Citronellolc | 21.312 | 26.916 | – | 0.824 | – | 0.846 |
| 33 | Bornyl acetate | 23.812 | 29.552 | 2.241 | 3.212 | 2.991 | 3.261 |
| 34 | Thymol | 24.108 | 29.794 | – | – | – | 0.666 |
| 35 | Carvacrol | 24.500 | 30.068 | 1.005 | 1.112 | 1.197 | 1.617 |
| 36 | Methyl eugenol | 28.964 | 34.339 | – | 1.040 | – | 1.169 |
| 37 | β -caryophyllene | 29.478 | 35.195 | 2.669 | 4.035 | 4.796 | 4.895 |
| 38 | α -humulene | 30.886 | 36.534 | 0.169 | 0.481 | 0.652 | 0.850 |
| 39 | Methyl jasmonate | 38.496 | 43.609 | – | 1.079 | – | 2.643 |
| % of total | 99.49 | 96.69 | 99.05 | 97.89 | |||
| Monoterpene hydrocarbons | 159.91 | 202.53 | 249.73 | 195.00 | |||
| Oxygenated compounds | 750.57 | 705.04 | 647.18 | 743.45 | |||
| Sesquiterpenes | 2.84 | 4.52 | 5.45 | 5.74 | |||
a RT 1: Retention time in min on HP-5MS column obtained by MSD;
b RT 2: Retention time in min on HP-5MS column obtained by FID
c Compounds determined using mass spectrum consistent with spectra found in the literature, but not using the retention time of standard compounds
(n = 6)