Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 May 26;205(4):493–510. doi: 10.1083/jcb.201310045

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Muslimov et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Dendritic transport of ID4-chimeric mRNA after β-adrenergic activation. (A and B) After β-adrenergic activation with the agonist isoproterenol, ID4-chimeric α-tubulin mRNA was delivered to distal dendrites of sympathetic neurons. (C and D) In contrast, after α-adrenergic activation with the agonist phenylephrine, ID4-chimeric α-tubulin mRNA was not transported to dendrites to any substantial extent. (E and F) Application of the β-AR antagonist propranolol before application of agonist isoproterenol blocked the isoproterenol-induced dendritic delivery of ID4-chimeric α-tubulin mRNA. Concentrations of agonists, antagonists, and other drugs are given in Materials and methods. Number of cells analyzed: (A and B) 16 cells, 52 dendrites; (C and D) 16 cells, 57 dendrites; (E and F) 14 cells, 50 dendrites. Bar, 50 µm. (G) Quantitative analysis. One-way ANOVA, Dunnett’s post hoc analysis (comparison of RNA levels in the basal state [Fig. 2] with RNA levels after adrenergic activation/blockade): comparison with phenylephrine (A and B), P > 0.7 for interval points 50 µm; comparison with isoproterenol (C and D), P < 0.001 for all interval points; comparison with isoproterenol/propranolol (E and F), P > 0.8 for interval points 50 µm. Error bars indicate SEM.