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. 2014 May 26;205(4):555–571. doi: 10.1083/jcb.201310018

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© 2014 Funk et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — D4 mediates Stu1 midzone association. (A–G) Cells were analyzed 2 h after G1 release. (A–D) D4 promotes Stu1 midzone positioning. Stu1 localization and anaphase spindle phenotypes were quantified as indicated. n > 40. (E) Stu1ΔD4 fails to localize to the midzone of intact anaphase spindles. Stu1ΔD4 was expressed in the WT background. n > 40. (F) D4 associates with the spindle midzone. Midzone localization of D4 was quantified in stu1ΔD4-ZIP cells with intact anaphase spindles as indicated. n > 100. (G) Localizing TOGL2 to the spindle midzone via D4 rescues the stu1ΔD4 spindle defect. Anaphase spindle phenotypes were quantified in stu1ΔD4 cells expressing the indicated constructs. n > 30. DIC, differential interference contrast. Bars, 2 µm.