Table 1.
Mutations in mus-101 suppress par-4 lethality
| Genotype | 18°C | 20°C | 25°C |
| % | % | % | |
| Wild type; ctl(RNAi)1 | 100 ± 0 (19) | 99.9 ± 0 (4) | 98.6 ± 0.4 (4) |
| Wild type; mus-101(RNAi) | 99.9 ± 0 (19) | 99.9 ± 0 (4) | 82.4 ± 2.1 (3) |
| lin-11(+) mus-101(tm1761)/lin-11(n389) mus-101(+); ctl(RNAi)2 | 100 ± 0 (4) | ND | ND |
| lin-11(+) mus-101(tm1761)/lin-11(n389) mus-101(+); mus-101(RNAi) | 100 ± 0 (4) | ND | ND |
| par-4(it47ts); ctl(RNAi) | 13.0 ± 0.8 (19) | 1.0 ± 0.6 (3) | 0 ± 0 (3) |
| par-4(it47ts); mus-101(RNAi) | 51.9 ± 0.8 (19)3 | 19.9 ± 0.8 (3)3 | 0 ± 0 (3) |
| par-4(it47ts); lin-11(+) mus-101(tm1761)/lin-11(n389) mus-101(+); ctl(RNAi) | 33.3 ± 0.9 (3)3 | ND | ND |
| par-4(it47ts); lin-11(+) mus-101(tm1761)/lin-11(n389) mus-101(+); mus-101(RNAi) | 74.5 ± 4.5 (3)3 | ND | 0 ± 0 (3) |
The values correspond to the mean percentage of hatching embryos over the total number of embryos ± SEM. The numbers in parentheses correspond to the number of triplicate assays performed. N.D., not determined.
1
Empty vector was used for RNAi control.
2
The gene lin-11 is positioned very close to the mus-101 gene on chromosome I, and heterozygote animals give rise to sterile adults (mus-101(tm1761) homozygotes), vulvaless adults (lin-11(n389) homozygotes), and viable, fertile heterozygotes.
3
The value is significantly different from the par-4 control at the same temperature (P < 0.05, Student’s t test).