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. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jan 23;124:632–637. doi: 10.1016/j.saa.2014.01.069

Table 2.

Binding constants Ka [M−1] and mean number of PCB molecules bound to the independent class of HSA binding sites (n) determined for HSA-PCB complexes using the Scatchard method and non-linear regression model, λex 295 and λex 280 nm.

λ ex Complex Ka (Scatchard) [M−1] n Ka (non-linear regression) [M−1] n
295 nm HSA-PCB118 2.150×104 1.89 2.182×104 0.70
HSA-PCB126 1.744 ×104 1.33 1.998×104 0.99
HSA-PCB153 1.511×104 1.47 1.652×104 0.88
280 nm HSA-PCB118 4.730 ×104 1.52 4.922×104 0.72
HSA-PCB126 6.291 ×104 1.31 7.180×104 0.79
HSA-PCB153 2.627 ×104 1.77 2.749×104 0.86