Table 1.
Primers designed for microarray data confirmation by quantitative PCR (qPCR).
| Primer | Sequence (5′ → 3′) | Concentrations | Amplicon |
|---|---|---|---|
| (nM) | size (bp) | ||
| SG0845F | GCCAGCCTTATGTGGAAGGT | 600 | 140 |
| SG0845R | AGCCGGGGTGACTTTAGTTG | 600 | |
| SG0858F | GTTCATTCTCGGTCTGCCCA | 600 | 136 |
| SG0858R | GGCGGGTAAGCCGACATATT | 600 | |
| SG0895F | CGACCTGGTTATTAGCGGCA | 300 | 121 |
| SG0895R | CGCTACGTTATCCACCGACA | 900 | |
| SG1978F | ACCACTGCAACCGTTTTTCG | 300 | 153 |
| SG1978R | GCAGCTTATGACCGTCTCGT | 150 | |
| Gmm Tub F | CCATTCCCACGTCTTCACTT | 600 | 149 |
| Gmm Tub R | GACCATGACGTGGATCACAG | 600 |
Primers were designed with Primer-Blast software. The third and fourth columns represent forward and reverse primer concentration used in qPCR and PCR product size obtained, respectively.