| Reagent | Volume (μl) per reaction | Final concentration (vol = 20 μl) |
|---|---|---|
| 5 x First strand buffer | 4 μl | |
| 0.1 M DTT | 1 μl | 5 mM |
| RNAsin | 1 μl | 2 U μl−1 |
| Mix total volume | 6 μl | |
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