| Step | Temperature | Time | Number of cycles |
|---|---|---|---|
| Initial denaturation | 95°C | 2 min | 1 |
| Denaturation | 98°C | 20 sec | |
| Annealing | 52°C | 20 sec | 19-24 |
| Extension | 68°C | 20 sec | |
| Final extension | 72°C | 5 min | 1 |
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