Reagent | Volume (μl) per reaction | Final concentration |
---|---|---|
Water | 57 μl | |
5 x PNK buffer | 16 μl | |
100 mM rATP | 0.8 μl | 1 mM |
RNAsin | 2 μl | 1 U μl−1 |
T4 PNK | 4 μl | 0.5 U μl−1 |
Mix total volume | 80 μl |
Reagent | Volume (μl) per reaction | Final concentration |
---|---|---|
Water | 57 μl | |
5 x PNK buffer | 16 μl | |
100 mM rATP | 0.8 μl | 1 mM |
RNAsin | 2 μl | 1 U μl−1 |
T4 PNK | 4 μl | 0.5 U μl−1 |
Mix total volume | 80 μl |