| Reagent | Volume (μl) per reaction | Final concentration |
|---|---|---|
| Water | 118.4 μl | |
| 5 x PNK buffer | 32 μl | |
| 100 mM rATP | 1.6 μl | 1 mM |
| RNAsin | 4 μl | 1 U μl−1 |
| T4 RNA ligase 1 | 4 μl | 0.25 U μl−1 |
| Mix total volume | 160 μl | |
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