| Reagent | Volume (μl) per reaction | Final concentration |
|---|---|---|
| Water | 54 μl | |
| 5 x PNK buffer | 16 μl | |
| RNAsin | 2 μl | 1 U μl−1 |
| TSAP | 8 μl | 0.1 U μl−1 |
| Mix total volume | 80 μl | |
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