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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Nat Protoc. 2014 Feb 27;9(3):711–728. doi: 10.1038/nprot.2014.043
Reagent Volume (μl) per reaction Final concentration
Water 18 μl
5 x PNK buffer 16 μl
25 % PEG 8000 32 μl 10 %
10 μM miRCat-33 3′-linker 8 μl 1 μM
RNAsin 2 μl 1 U μl−1
T4 RNA ligase 2, truncated, K227Q 4 μl 10 U μl−1
Mix total volume 80 μl