| Reagent | Volume (μl) per reaction | Final concentration |
|---|---|---|
| Water | 55 μl | |
| 5 x PNK buffer | 16 μl | |
| 32P-γ-ATP | 3 μl | |
| RNAsin | 2 μl | 1 U μl−1 |
| T4 PNK | 4 μl | 0.5 U μl−1 |
| Mix total volume | 80 μl | |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.