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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Nat Protoc. 2014 Feb 27;9(3):711–728. doi: 10.1038/nprot.2014.043
Reagent Volume (μl) per reaction Final concentration
T4 RNA ligase reaction buffer, 10× 1.5 μl
10 mM ATP 1.5 μl 1 mM
T4 PNK 1 μl 0.67 U μl−1
Water 11 μl
Mix total volume 15 μl