| Reagent | Volume (μl) per reaction | Final concentration (vol = 20 μl) |
|---|---|---|
| Water | 8 μl | |
| 2.5 mM dNTPs | 4 μl | 0.5 mM |
| 10 μM miRCat-33 primer | 1 μl | 0.5 μM |
| Mix total volume | 13 μl | |
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