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. 2014 May 27;5:157. doi: 10.3389/fgene.2014.00157

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Copyright © 2014 Paszkiewicz, Farbos, O'Neill and Moore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Estimating required read sampling for contaminant checks. Ten million reads from an Illumina RNA-seq dataset was subsampled at various numbers of reads. The number of rRNA contaminant reads in this dataset was 1.86% when calculated over the full dataset. The absolute percentage difference at different sub-sample sizes was calculated for 500 replicates at each depth and the average shown. The error bars indicate the 95% confidence interval for the absolute percentage difference.