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. 2014 May 27;5:236. doi: 10.3389/fimmu.2014.00236

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Copyright © 2014 Cohen, Parada, Acosta-Gío and Espitia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Humoral immune response to PE_PGRS33, PGRS, and PE in immunized mice. BALB/c mice were immunized with PE_PGRS33 and sera were collected 15 days after the last immunization. IgG (A), IgG1 (B), and IgG2a (C) responses to PE_PGRS33 (□), PE (▲), PGRS (○), and the control protein GST (♦), were evaluated by ELISA assay. For total IgG determination, each serum was diluted 1:100 and for IgG1 and IgG2a determination, mice sera were diluted as indicated in the figure. Bars (A) and datum points (B,C) are the average readings ± SD of data from four mice in the group, and the results showed are representative of three independent experiments.