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Antigen-specific IFN-γ secretion in immunized mice. Spleen cells from mice immunized with PE_PGRS33 or from control mice were incubated with 25 μg of PE_PGRS33, PGRS, PE, or GST recombinant purified proteins plus 10 μg/ml of polymyxin B for 4 days. The culture supernatants were evaluated for IFN-γ production using ELISA assay. Each bar represents the mean ± SD of data from four mice per group. Representative results of three independent experiments are shown.
