Table 3.
Calcium retention capacity of isolated mitochondria. Freshly prepared mitochondria from mouse liver were incubated with increasing concentrations of olesoxime or CsA for 5 minutes prior to the start of calcium addition. Pulses of 10 µM calcium were added and Calcium Green fluorescence was measured 50 seconds after addition to measure extramitochondrial calcium concentration until there was no further mitochondrial uptake. The number of calcium pulses prior to loss of calcium uptake was used to calculate the mitochondrial calcium retention capacity. Data represent mean ± SD (2 independent experiments run in triplicate each).
| μM | Control | Olesoxime | CsA | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 0.1 | 0.5 | 1 | 5 | 0.1 | 0.5 | 1 | 5 | ||
| mPTP induction (μM Ca2+) | 34.5 | 36.6 | 37.9 | 35.4 | 35.7 | 71.4 | 80.7 | 85.3 | 87.7 |
| SD | 1.72 | 0.80 | 2.28 | 1.04 | 0.57 | 1.12 | 3.48 | 1.25 | 2.71 |
| Norm. vs control | 1 | 2.1 | 3.4 | 0.8 | 1.2 | 36.9 | 46.2 | 50.8 | 53.1 |