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. 2010 Mar 30;3(4):961–993. doi: 10.3390/ph3040961

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© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Efficiency of transfection mediated by different complexes containing the S4₁₃-PV peptide. HeLa cells were incubated with free plasmid DNA, cationic liposome/DNA complexes, Lipofectamine 2000-based complexes and the ternary complexes for 4 h at 37 ºC. Transfection efficiency was evaluated, 48 h later, by flow cytometry analysis of GFP expression and the percentage of GFP-positive cells is presented. Ternary complexes were obtained by the addition of cationic liposomes composed of DOTAP:DOPE to complexes of S4₁₃-PV, prepared at different peptide/DNA charge ratios. Ternary complexes were able to transfect cells more efficiently than cationic liposome/DNA complexes and at similar levels than those obtained with Lipofectamine 2000.