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. 2014 May 27;2:31. doi: 10.3389/fchem.2014.00031

Figure 4.

Figure 4

Osteogenic differentiation of BMSCs on TMV and TMV-RGD substrates. (A) Osteo-specific gene expression profiles of BMSCs on TMV and TMV-RGD under osteogenic conditions over 21 days. For each sample, the profiles show 3 time points at 7, 14, and 21 days. BMSCs on TMV-RGD substrates have significantly higher BGLAP at day 14 and IBSP at day 21 when compared with unmodified TMV substrates. Error bars indicate ±1 SD. *p < 0.05. (B) Alkaline phosphatase activity of cells on TMV and TMV-RGD at day 14. BMSCs on TMV-RGD substrates have a slight increase in enzyme activity compared to cells on TMV. However, the difference is not statistically significant. (C) Optical images of Alizarin red S staining of cells cultured for 14 days on TMV or TMV-RGD substrate showing calcium deposition in red colour.