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Modification of native bovine serum albumin by 14C-iodoacetamide. (A) MS/MS relative peptide abundance total ion chromatogram. (B) Peptide fraction collection and SS-AMS analysis. (C) LS-AMS 12C trace in units of ng 12C/measurement interval (top) and LS-AMS 14C trace in units of amol 14C/measurement interval (bottom). 14C peak 3 is due to the peptide containing Cys 58, GLVLIAFSQYLQQCPFDEHVK. Minor 14C peaks 1 and 2 contain multiple Cys-containing peptides.
