Figure 2.
Rapid Ca2+ fluxing in CD3/CD28 costimulated PBL from 19 lupus and 11 control donors. PBL were preloaded with Fluo-3 and stimulated with OKT3 and anti-CD28 mAbs while Fluo-3 fluorescence of CD3+/annexinV− T cells was continuously recorded by flow cytometry. Results are expressed as RF values with respect to those of unstimulated cells normalized at 1.0. Data present mean ± SE. Numbers over each time point indicate p values reflecting differences between lupus and control donors.