Fig. 2.
The diffusive behavior of WT and wedge mutant DNA glycosylases on undamaged λ DNA. Representative displacement vs. time traces for (A) Nei and (B) Nei Y72A. Overall diffusion constants (Dtraj) are determined by linear fits to the first 25% of the MSD plots (Insets; error bars indicate SEM). Sliding window diffusion constant Dint for representative traces of (C) Nei and (D) Nei Y72A show the time course of diffusive behavior, showing that the wedge mutant shows overall faster diffusion and an absence of periods of little to no motion. Ordinate tick marks indicate powers of 10. Dint distributions for (E) Fpg, (F) Nei, and (G) Nth WT enzymes (filled symbols) relative to their respective wedge mutants (open symbols) (and see also Fig. S2). (H) Mean overall diffusion constant Dtraj of variants (dark bars) of Fpg, Nei, and Nth are significantly faster than the WT versions of the enzymes (light bars; ***P < 0.001). See also Movies S1 and S2.