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. 2014 May 5;111(20):7331–7336. doi: 10.1073/pnas.1406898111

Fig. 1.

Fig. 1.

VCP requirement for dendrite pruning is linked to ecdysone signaling. (A) Morphology of the dorsal class IV da neuron ddaC at the third instar larval stage. (B) ddaC morphology at 16 h APF. All larval dendrites are pruned (0/38 with dendrites attached to soma). (C) vcp26-8 mutant ddaC neurons at 16 h APF show severe defects in dendrite pruning (8/8 attached to soma, P < 0.005). (D) Expression of dominant-negative VCP QQ in class IV da neurons inhibits dendrite pruning at 16 h APF (18/30 with dendrites attached to soma, P < 0.005). (E and F) Overexpression of the ecdysone pathway transcription factor Sox14 suppresses the pruning defects induced by VCP QQ. (E) Sox14 overexpression does not affect dendrite pruning at 16 h APF (0/34). (F) Sox14 overexpression suppresses dendrite pruning defects induced by VCP QQ (0/50). Statistical comparison of attached versus severed dendrites was with Fisher’s exact test. (Scale bars in A and B, 20 μm.) Arrowheads denote the positions of class IV da neuron cell bodies.