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. 2014 May 5;111(20):7331–7336. doi: 10.1073/pnas.1406898111

Fig. 3.

Fig. 3.

MICAL mRNA is misspliced upon VCP inhibition. Total RNA was isolated from control class IV da neurons or class IV da neurons expressing VCP QQ at 1–5 h APF and analyzed by RT-PCR. (A) Schematic representation of part of the MICAL gene. The large arrow indicates the direction of transcription and the small arrows the positions of RT-PCR primers. The exon numbering is as on flybase.org. (B and C) RT-PCR reactions from control or VCP QQ-expressing samples: (B) MICAL exons 14–16 and (C) MICAL exons 8–12. (D) Schematic representation of change in MICAL splicing upon VCP inhibition. The stop codon induced by VCP QQ would delete 1,612 amino acids from the Mical protein compared with the full-length protein.