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. 2014 Apr 30;111(20):7373–7378. doi: 10.1073/pnas.1322275111

Fig. 3.

Fig. 3.

Hipk2 ablation in vivo is associated with impaired white adipose tissue development and browning/beigeing of WAT depots. (A) Representative abdominal images of 8-wk-old female Hipk2+/+ and Hipk2−/− mice. (B) Weight of adipose depots (o, ovarian; m, mesenteric; r, retroperitoneal) from 8- to 10-wk-old female Hipk2+/+ (n = 8) and Hipk2−/− (n = 8) mice after normalization to body weight. (C) Consecutive sections of ovarian WAT from Hipk2+/+ and Hipk2−/− 8-wk-old female mice stained with H&E or with antibodies against the general adipocyte marker perilipin (green), the brown adipocyte marker Ucp1 (red), and the beige adipocyte markers Tmem26 (red) and Cited1 (red). Nuclei were counterstained with DAPI (blue). White arrows denote blood vessels in serial sections. (Scale bars, 100 μm.) (D) mRNA levels of Pparg1/2, Pparg2, Cebpa, Cebpb, Fabp4, Mest, Cidea, Ppargc1a, and Ucp1 in ovarian WAT of 8- to 10-wk-old female Hipk2+/+ (n = 4) and Hipk2−/− (n = 5) mice. *P < 0.05, **P < 0.01, and ***P < 0.001 for Hipk2+/+ versus Hipk2−/− mice. Data are presented as mean ± SEM.