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Naïve T cells display basal NF-κB activity. (A) EMSA for NF-κB using nuclear extracts of WT and IκBαΔN FACS cell-sorted CD4+CD44lo and CD8+CD44lo T cells. (B) EMSA supershift for NF-κB subunits RelA and p50 performed with nuclear extracts from WT naïve T cells. Results are representative of at least three independent experiments. †Supershifted bands for RelA. ‡Supershifted band for p50.
