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. 2012 Oct 25;31(4):77–84. doi: 10.12938/bmfh.31.77

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Bioscience of Microbiota, Food and Health

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 4. — OVA-specific IgG1 levels in the culture supernatant of splenocytes isolated from BALB/c mice with or without C. albicans gut colonization. Mice fed OVA (Oral OVA (+)) or PBS (Oral OVA (–)) were intraperitoneally immunized with OVA in alum. The spleen was removed three weeks after immunization, and splenocytes isolated from individual mice were pooled in each group and then subjected to an ex vivo antibody production assay. Panel A shows the effect of ex vivo stimulation by OVA (100 μg/ml), and panel B shows the effect of ex vivo supplementation with heat-killed C. albicans (Candida) and the culture supernatant of C. albicans (SUP) prepared in the yeast (Y) and hyphal (H) forms. Values are presented as means ± SEM of three wells.