Table 2. Effect of high amylose cornstarch on H₂ concentration and aminotransferase activities in the portal vein, and urinary 8-OHdG and allantoin concentrations in IR-treated and sham-operated rats.

	Sham		IR		Two-way ANOVA
	C	HAS	C	HAS	Diet	Treatment	Interaction
Portal
H2 (mmol/L)	0.780ab(0.197–5.70)	3.16b(0.374–17.7)	0.353a(0.087–2.35)	2.57b(0.345–16.8)
Plasma ALT (mkat/L)	0.159a(0.082–0.352)	0.175a(0.090–0.243)	0.384b(0.096–2.68)	0.229ab(0.046–0.992)
Plasma AST (mkat/L)	1.46ab(1.14–2.43)	1.50a(1.32–1.82)	1.84b(1.45–9.10)	1.39ab,*(0.95–4.96)
Urine
8-OHdG (mmol/mol creatinine)
Days 10–12	8.02 ± 1.18	10.4 ± 2.4	8.71 ± 1.35	12.5 ± 2.6	0.1218	0.4775	0.7190
Days 12–14	11.2 ± 1.9	9.70 ± 2.30	11.6 ± 2.6	13.8 ± 4.4	0.9075	0.4510	0.5390
Allantoin (mol/mol creatinine)
Days 10–12	3.56 ± 0.17	3.60 ± 0.17	3.54 ± 0.09	3.60 ± 0.11	0.7223	0.9616	0.9416
Days 12–14	4.47 ± 0.12	4.83 ± 0.24	4.66 ± 0.11	4.85 ± 0.13	0.0943	0.5175	0.5901

ALT, alanine aminotransferase; AST, aspartate aminotransferase; C, control; HAS, high amylose cornstarch; IR, ischemia-reperfusion; H₂, hydrogen; 8-OHdG, 8-hydroxy-2'-deoxyguanosine. High H₂ generating rats, which we selected, were used. After 30 min of ischemia, 45 min of reperfusion was performed in IR rats. Values are expressed as means ± SEM or medians (minimum value-maximum value), n=12–13. Data were analyzed with two-way ANOVA and Tukey-Kramer’s post hoc test or nonparametric multiple test (Steel-Dwass test). Mean values within a row with different superscript letters are significantly different (p < 0.05).

* p < 0.05 compared with the IR-C group. Values of plasma ALT and AST activities in both IR groups were analyzed by Mann-Whitney’s U test in case of the variances that were too different between the sham-operated and IR treated groups.