Table 3. Effect of high amylose cornstarch on hepatic glutathione and MDA levels and SOD and MPO activities in IR-treated and sham-operated rats.

	Sham		IR		Two-way ANOVA
	C	HAS	C	HAS	Diet	Treatment	Interaction
Glutathione
Total (mmol/g tissue)	9.78 ± 0.19	10.0 ± 0.3	9.96 ± 0.30	10.5 ± 0.3	0.1664	0.2441	0.6182
GSH (mmol/g tissue)	9.45 ± 0.20	9.62 ± 0.25	9.64 ± 0.31	10.1 ± 0.3	0.2217	0.1893	0.5356
GSSG (mmol/g tissue)	0.326 ± 0.027	0.405 ± 0.047	0.318 ± 0.026	0.336 ± 0.036	0.1701	0.2749	0.3756
GSH/GSSG	30.9 ± 2.4	26.7 ± 2.4	32.2 ± 2.3	32.6 ± 2.3	0.4174	0.1306	0.3396
SOD activity
Total (U/g tissue)	8660 ± 340	7160 ± 820	8400 ± 840	7350 ± 670	0.0752	0.9575	0.7488
Mn type (U/g tissue)	417 ± 66	262 ± 38	370 ± 120	279 ± 38	0.8435	0.1124	0.6759
Mn type/Total	0.0488 ± 0.0080	0.0378 ± 0.0046	0.0464 ± 0.0153	0.0384 ± 0.0047	0.3281	0.9213	0.8775
MDA (nmol/g tissue)	135 ± 11	152 ± 9	148 ± 8	151 ± 10	0.3219	0.5575	0.4786
MPO (U/g tissue)	0.0247 ± 0.0042	0.0326 ± 0.0069	0.112 ± 0.064	0.153 ± 0.048	0.5625	0.0167	0.6954

C, control; HAS, high amylose cornstarch; IR, ischemia-reperfusion; GSH, reduced glutathione; GSSG, oxidized glutathione; SOD, superoxide dismutase; MDA, malondialdehyde; MPO, myeloperoxidase. High H₂ generating rats, which we selected, were used. After 30 min of ischemia, 45 min of reperfusion was performed in IR rats. Values are expressed as means ± SEM, n=12–13. Data were analyzed with two-way ANOVA. Total glutathione and GSSG concentrations were expressed as GSH equivalent.