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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Feb 4;69:88–96. doi: 10.1016/j.yjmcc.2014.01.016

Figure 2.

Figure 2

Localization of cytoskeletal elements and migratory characteristics differ between cells derived from embryonic aAo versus dAo. A–C) Embryonic aAo VSMCs labeled with anti-vinculin antibody and phalloidin. D–F) Cells derived from the embryonic dAo labeled with anti-vinculin antibodies and phalloidin. DNA labeled with DAPI. G) Quantification of relative focal adhesion area. H, I) Scratch assay using embryonic aAo and dAo VSMC lines, 6 hours after scratch was applied. J) Quantification of percent wound closure in embryonic VSMC lines. K, L) Boyden chamber assay on embryonic VSMCs t=24 hours. Cells stained with Giemsa demonstrate the number of cell that invaded the membrane. M) Quantification of number of cells migrated after 24 hours. Scale bars represent 50µm. Abbreviations: Vin, vinculin; Phal, phalloidin. Error bars represent SEM. *, p≤0.05; **, p≤0.01.