Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Feb 4;69:88–96. doi: 10.1016/j.yjmcc.2014.01.016

Figure 5.

Figure 5

VSMC lines from adult aAo and dAo have similar localization of cytoskeletal elements and migratory characteristics. A–C) Representative adult aAo VSMCs labeled with anti-vinculin antibody and phalloidin. D–F) Representative adult dAo VSMC lines labeled with anti-vinculin antibodies and phalloidin. DNA labeled with DAPI. G) Quantification of relative focal adhesion area. H, I) Scratch assay of adult aAo and dAo VSMC lines after 6 hours. J) Quantification of percent wound closure in adult VSMC cultures. K, L) Boyden chamber assay on adult VSMC lines t=24 hours. M) Quantification of number of cells migrated after 24 hours. Scale bars represent 50µm. Abbreviations: aAo, ascending aorta; dAo, descending aorta; Vin, vinculin; Phal, phalloidin. Error bars represent SEM. *, p≤0.05; **, p≤0.01.