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. Author manuscript; available in PMC: 2014 May 27.
Published in final edited form as: Nature. 2013 Aug 14;500(7464):598–602. doi: 10.1038/nature12451

Figure 3. PCGEM1 and PRNCR1 Promote Hormone-independent Activation of the AR Transcriptional Program in Castration Resistant Prostate Cancer (CRPC).

Figure 3

a, qRT-PCR analyses of AR targets in LNCaP cells co-transfected with indicated vectors followed by Doxycycline induction (160 ng/ml, 2d). b, RIP assay in CWR22Rv1 cells treated with or without DHT (100 nM, 1hr) using indicated antibodies. Detection of immunoprecipitated full-length AR and AR-V7 were shown. c, Cell proliferation assay in CWR22Rv1 cells stably expressing indicated shRNAs followed by Doxycycline induction (160 ng/ml) for indicated times. d, Xenografts of CWR22Rv1 cell lines harboring Doxycycline-induced shRNAs were monitored for tumor growth for the indicated time, with or without Doxycycline intake (4 mice/group). Mean ± SEM (n=6, *p<0.05 and **p<0.01).