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. 2014 May 8;2014:470482. doi: 10.1155/2014/470482

Figure 10.

Figure 10

FLAG-tagged ref(2)P coprecipitates with both HA-Atg101 and HA-Atg8a, but not with anti-HA beads (a). Note that HA-Atg8a-II migrates faster than HA-Atg8a-I, due to the covalent attachment of the lipid moiety phosphatidylethanolamine to its C-terminus. (b) The selective autophagy cargo ref(2)P coprecipitates with Atg8a, Atg101, Atg18, RagC, Atg13, Atg16, and Keap1, but not with empty beads.