Figure 3.

Knockdown of Atg101 using separate RNAi lines ((a), (b)), or the combination of both (c), impairs the formation of Atg8a-positive autophagosomes upon starvation. Note that fewer but bigger Atg8a puncta are seen in Lamp1-GFP-marked RNAi cells than in neighboring control fat body cells. (d) Quantification of data shown in (a) to (c), N = 5, P < 0.001, two-tailed, two-sample Student's t-tests. (e) Overlapping Atg8a and ref(2)P structures are highlighted by arrowheads in Atg101 depleted cells. Scalebar equals 20 μm.