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. 2014 May 27;5:224. doi: 10.3389/fmicb.2014.00224

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Copyright © 2014 Elshawadfy, Keith, Ee Ooi, Kinsman, Heslop and Connolly.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Elongation of Primer-templates by archaeal family-B DNA polymerases. Gel electrophoretic analysis of primer strand extension observed with: (A) Pfu-Pol wild type; (B) Pfu-Pol M247R/L381R; (C) Pfu-Pol L381R/K501R; (D) Tkod-Pol. The primer-template used was: 5′-GGGGATCCTCTAGAGTCGACCTGC 3′-CCCCTAGGAGATCTCAGCTGGACGACCGTTCGTTCGAACAGAGG. The primer was labeled at its 5′-terminus with either cyanine 5 (used with wild type Pfu-Pol and the double mutants) or fluorescein (used with Tkod-Pol).