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. 2014 Jun;141(11):2245–2254. doi: 10.1242/dev.103960

Fig. 4.

Fig. 4.

Icam4 is directly regulated by EKLF. (A) Data from the mouse Erythron database (Kingsley et al., 2013) showing changes in ICAM4 and EKLF (KLF1) RNA levels during primitive and definitive (fetal liver or bone marrow) erythroid differentiation. (B) qRT-PCR analysis of ICAM4 levels in E13.5 fetal liver cells. Comparison was made between wild type (+/+), EKLF-het (+/−), or EKLF-null (−/−) as indicated. Normalization is to GAPDH. (C) Genome browser data (http://main.genome-browser.bx.psu.edu/) indicating ICAM4 transcript levels in primary erythroblasts, and overlap between KLF1, TAL1, GATA1 and P300 ChIP data at the 5′ Icam4 promoter. Also indicated are the locations of matches to the KLF1 consensus target site 5′-CCMCRCCCN-3′ (Siatecka et al., 2010; Tallack et al., 2010). B, basophilic erythroblast; O, orthochromatic erythroblast; P, proerythroblast; R, reticulocyte.