Table 2.
Tm and extension time for amplifying the corresponding segment.
| PCR fragments | Annealing temperature (°C) | Extension time (S) |
|---|---|---|
| Frag-1 | 56 | 30 |
| Frag-2 | 58 | 60 |
| Frag-3 | 58 | 60 |
| Frag-4 | 58 | 90 |
| Nsp2-GFP frag | 60 | 120 |
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