Table 1.

Effects of experimental parameters on laccase immobilization yield on perlite. NaC: 50 mM sodium citrate buffer; McI: McIlvaine's buffer. *Incubations were performed in 50 mM sodium phosphate buffer, unless otherwise is indicated. Reported data are the mean values of three independent experiments. The standard deviation of tests carried out at a given set of operating conditions was less than ±10%.

Run	Initial activity (IU/g)	Initial protein (mg/g)	Glutaraldehyde % vol	pH*	Incubation time and temperature	Immobilized activity (IU/g)	Immobilization yield (%)
R1	550	4.5	1	6.5	4 h RT	200	36
R2	550	4.5	1	6.5	Overnight 4°C	128	23
R3	550	4.5	2.5	6.5	4 h RT	206	37
R4	550	4.5	2.5	6.5	Overnight 4°C	151	27
R5	550	4.5	5	6.5	4 h RT	206	37
R6	550	4.5	5	6.5	Overnight 4°C	207	38
R7	550	7.75	1	6.5	1 h RT	160	29
R8	550	7.75	1	6.5	4 h RT	161	29
R9	550	7.75	0.5	6.5	1 h RT	186	34
R10	550	7.75	0.5	6.5	4 h RT	178	32
R11	80	1.3	0.5	6.5	1 h RT	56	70
R12	285	4.75	0.5	6.5	1 h RT	136	48
R13	800	11.25	0.5	6.5	1 h RT	199	25
R14	500	4.25	0.5	6.5	1 h RT	173	35
R15	500	12	0.5	6.5	1 h RT	173	35
R16	500	22.5	0.5	6.5	1 h RT	184	37
R17	275	2.25	0.5	NaC 5.5	1 h RT	52	19
R18	275	2.25	0.5	6.5	1 h RT	137	50
R19	275	2.25	0.5	7.5	1 h RT	120	43
R20	275	2.25	0.5	McI 5.5	1 h RT	32	12
R21	275	2.25	0.5	McI 7.5	1 h RT	66	24
R22	275	2.25	0.5	McI 6.5	1 h RT	42	15