(A) Primary lung AECs from FLCNf/f mice were infected with control (−) or Cre- recombinase-expressing adenovirus (AdCre,+) followed by immunoblot analysis. See also Figure S3.
(B) Immunoblot analyses of mouse epithelial NMuMG cells transfected with Flcn siRNA (siFlcn) or control scrambled siRNA (−).
(C) Re-expression of FLCN in human FLCN-null UOK-257 cells increases membrane localization of LKB1.
Top: representative images. Bottom: statistical analyses. Protein ratio for control cells were taken as one fold. Data are mean ± SE, n=3.