(A) Flcn knockdown downregulates membrane localization of E-cadherin (red). Scale bars, 25 μM.
See also Figure S5 and S6.
(B) Flcn knockdown decreases trans-epithelial resistance (TER). TER of siContr-transfected NMuMG cells was taken as 100%.
(C) Cleaved caspase-3 is upregulated by siFlcn.
(D) Flcn knockdown induces DNA fragmentation (TUNEL assay) of NMuMG cells.
(E) AICAR and constitutively active AMPK (AMPK-CA) rescue disruption of epithelial cell morphology caused by siFlcn. Cells were treated either with 100 mM AICAR or diluent, or were infected with AdAMPK-CA or control adenovirus. Scale bars, 100 μM.
(F–G) Expression of AMPK-CA (F) and AICAR-induced AMPK and ACC (G) phosphorylation in epithelial NMuMG cells.
(H–I) DNA fragmentation (H) and epithelial cell death (I) induced by Flcn loss is rescued by AICAR and AMPK-CA. Data represent percentage of TUNEL-positive (H) or dead (I) cells per total number of cells taken as 100%.
Data are mean ± SE, n=3.