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. Author manuscript; available in PMC: 2014 May 27.
Published in final edited form as: Nat Protoc. 2009;4(5):767–782. doi: 10.1038/nprot.2009.40

Figure 3.

Figure 3

Retrieval of cells from microwells with a micromanipulator.

(a) Phase-contrast image of a section of a microwell array with a 20-µm-diameter GC-1 capillary tip (Narishige), attached to an IM-9A micromanipulator (Narishige), inserted into a well loaded with cells.

(b) Phase-contrast image of the microwell array section in a, with the cells selectively removed from the central microwell. (c) The cells recovered from the central microwell in a were transferred into a well of a 96-well plate containing cloning media, and cultured until confluent. Note that the design of the array of microwells used here differs from the example shown in Figure 2. Scale bar, 100 µm.