Figure 6. PG depletion from impacted cartilage and the correlation to MMP-3 expression.
A) The contents of sGAG in medium samples collected daily till the 9th day post-impact were measured with DMMB assay. B) Comparison of MMP-3 up-regulation by soluble factors from the lower-energy-impacted cartilage to non-impacted controls. C) Comparison of MMP-3 up-regulation by soluble factors from the higher-energy-impacted cartilage to non-impacted controls. S. F. M. = serum free media. *The loading vol. was reduced to half of the others due to saturated MMP-3 signal.