Table 1.
Apoptotic index, Johnsen scores, and caspase-3 activities in different groups (mean ± SD).
| Apoptotic index (%) | Johnsen scores | Caspase-3 activity (absorbance 405/100 μg protein) |
|
|---|---|---|---|
| Sham | 8.50 ± 4.10** | 7.50 ± 0.80* | 0.10 ± 0.00** |
| Torsion | 32.40 ± 7.10 | 5.22 ± 1.21 | 0.62 ± 0.10 |
| T/D | 30.52 ± 10.20 | 5.50 ± 1.00 | 0.55 ± 0.05 |
| T/D + LXA4 (0.1 μg/kg) | 24.60 ± 7.70# | 5.80 ± 1.00# | 0.42 ± 0.12## |
| T/D + LXA4 (1.0 μg/kg) | 18.50 ± 8.12## | 6.22 ± 1.40## | 0.35 ± 0.08## |
| T/D + LXA4 (10 μg/kg) | 14.30 ± 6.75## | 7.15 ± 1.50## | 0.22 ± 0.05## |
T/D: torsion/detorsion; LXA4: lipoxin A4. Testicular injury and spermatogenesis were graded with Johnsen score. All tubular sections in each observed area of testicular tissue are evaluated systematically and each is given a score from 1 to 10. Complete spermatogenesis with many spermatozoa present is evaluated as score 10. Compared with other groups, *P < 0.05 and **P < 0.01. Compared with torsion or torsion/detorsion (T/D) group, # P < 0.05 and ## P < 0.01.